6. Service Contracts, Preventative Maintenance and Performance. ) at 490 nm. One plate carrier adapter is provided with the instrument. Description. 10. well, and strip-well microplates. TDP-43 stress granular cells were treated with 70% ice-cold. 00 USD. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. Endpoint, kinetic, spectrum. (Shiga, Japan) and kept for 30 min in the dark. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The area under the curve (AUC) for the comparison of the growth rates was determined using. 316 grating motor driver faulty. SPECTRAMAX 340. 3. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 0 OD. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. 1: SpectraMax Plus 384 components. SpectraMax ® Plus 384 optics. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. 4. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 303 unable to cal dark current. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. using SpectraMax i3 (Molecular devices, San Jose, USA). All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. #N207) LAL Reagent Water (Lonza cat. Both readers feature an 8-channel read head for fast reads and. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. Manager: Yinghua Chen, PhD Contact Email: yinghua. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Dissolve 13. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Male 8 pin mini Din to Female 9D. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. 5. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. Bioz Stars score: 86/100, based on 1 PubMed citations. , Sunnyvale, CA). MCE has not independently confirmed the accuracy of these methods. The assays contained 0. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 6. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. 5. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. Clotting times (mean ± SD) for rSimukunin and rSV. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. This was followed by an additional incubation at 37 °C for 30–60 min. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . ): 8. Values were determined according to a standard curve (log/log curve-fit). SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. A 0. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. 0% and ± 0. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Emission. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Or scan up to 384 wells at any time to determine the best assay conditions. Molecular Devices SpectraMax Gemini XS Microplate Reader. Photometric Range: 0. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. Unit powers. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. 30 µs. Donor filter: 616/10 nm. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Wavelength(s) 340 to 850 nm; Add to Compare List. A. 5% phosphoric acid) in microplates at room temperature for 10 min. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. The absorbances were transformed to velocity of substrate consumption (nmol/min). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Samples were plated in duplicate (r=0. Microplate Readers. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. In vivo anti-malarial assay in mice. , Sunnyvale, CA, USA) at 595 nm. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Our absorbance plate readers feature our PathCheck Sensor technology and. 313 reference gain check fail. MOLECULAR DEVICE M5. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Crowder, Ziqian Zeng, Alissa N. . PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 4; 100 m M NaCl, 0. 4. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Softmax PRO v5. reader (SpectraMax 340; Molecular devices, LLc). 04. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. 314 low lamp level warning. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. Call 1-800-745-2710. Call 1-800-745-2710. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. Each SpectraMax® 340 PC 384 Microplate Reader has an. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Subsequently, the mixture was heated at 100 °C for 10. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 190-1000 nm. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. #N207) LAL Reagent Water (Lonza cat. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. 2. Novak, Nathan J. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Analysis of ALA by Ehrlich’s Reagent Assay. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. Also for: Spectramax 190, Spectramax 340pc384, Versamax. 2. The. The plate was covered and incubated for one hour at room temperature. This assay measures the two major sugars in wine, glucose and fructose. 5 g/L-EDTA 0. These robust readers have been placed in labs from Antarctica to the. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). Awareness Technology, Inc. Calculating % of total mediator release in RBL assay. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Description 1. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). 2 software was used to measure and calculate of the bioactivity activity studies. 30. 1x10 5 transduced, GFP + SK-NEP-1. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 2. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 97), and each plate contained at least six positive and six negative controls. , San Jose, CA) using Softmax Pro 7 software. California Lightworks uses only the highest quality LEDs from Osram. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. StakMax. , Sunnyvale, CA). Read one sample or up to 384. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 0 2. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 5 mL of 1 M acetate buffer (pH 4. 2 M DEEMM were mixed in a microtube,. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. SpectraMax Microplate Readers. Acceptor filter: 665 nm. This reader only allows high performance HTRF measurement when assays are run in. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Immunofluorescence and Aggregation Analysis. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax Multi-Mode Microplate Readers. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Data were expressed as the mean LPS-treated cells (100%) vs. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). The ABS reader ranges from. Molecular Devices SpectraMAX 340 Microplate Reader. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Microplate Readers. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. 0. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Assay setup for a 384-well low-volume plate. Microplate Readers. Anti-proteolytic activity. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). SoftMax Pro. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Please refer to photos for further. 5), L-ascorbic acid (25. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Revvity. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. The initial rate of hydrolysis was determined using the early linear region of. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Example: [Drawer]. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Quick View. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. TDP-43 stress granular cells were treated with 70% ice-cold. Other technologies such as imaging. The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. The slope of the linear portion of the kinetic curve generated from each well was used to. Wavelength range: 340-850 nm. 5 stars - 1602 reviewsThis assay typically develops in 1. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 305 reference level saturation. Microplate Spectrophotometer Operator’s Manual. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. Amino Acid Analysis. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). To correct for inter. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. All strains were tested three times and the results averaged. Exhibit with us {{>currencyLabel}} Back. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. 81. 30. 8. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. used. Table 1. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. The adapter is. 305 reference level saturation. , Fremont, CA, USA). Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). . Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Samples containing 25 μg of protein were separated on 12. Wells were washed three times. 315 can't find zero order. Molecular Devices SpectraMax M5e Microplate Reader $26,500. 0200-6117. Data System. 340/70 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The growth. , Fremont, CA, USA). 1. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Products : SpectraMax Plus 384 reader,. Molecular Devices’ PathCheck® sensor technology. 6) and 0. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). 00 USD. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Assay setup for a 384-well low-volume plate. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). Molecular Devices Spectramax 340 Plate Reader Repair. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 2 - 3. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. using SpectraMax i3 (Molecular devices, San Jose, USA). Cet appareil ISM est confomre à la norme NMB-001 du Canada. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. SpectraMax Multi-Mode Microplate Readers. Male 8 pin mini Din to Female 9D. 30. MCE has not independently confirmed the accuracy of these methods. 5. Stat Fax 3200 Microplate Reader. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Molecular Devices. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. chen@case. Quick View. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The reaction mixture was incubated for 5 min. 1% N-1-naphtylethylenediamine dihydrochloride, 2. 5 mL of 1 M acetate buffer (pH 4. 0200-7027. Wavelength Selection. 7. The VersaMax and SpectraMax 190 read 96-well microplates. Warranty. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. The Catalina. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Integration delay. Cytotoxicity was determined by measuring the release of LDH. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 5. application or budget. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). 20. 313 reference gain check fail. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. Assay buffer was 50 m M Hepes, pH 7. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. SpectraMAX 340. Awareness Technology, Inc. D2. 340 nm. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Fibrin clots were formed as described above with some modifications 46. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). Microplate Spectrophotometers. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. The SpectraMax® iD3 Multi Mode Microplate . , Sunnyvale CA). SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. 3 0. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). SoftMax Pro. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. Create custom fluorescence assays without having to purchase expensive filter sets. FlexStation® 3 Multi-Mode Microplate Reader. Description 1. Data are expressed as percent of maximum binding for each protein. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates.